Semester V
MB306·Sem V·Practical
Recombinant DNA Technology Lab
2
Credits
0-0-4
L-T-P
1
Units
Key topics
- ▸Plasmid DNA isolation from E. coli
- ▸Restriction enzyme digestion
- ▸Agarose gel electrophoresis & fragment size estimation
- ▸Preparation of competent cells
- ▸Transformation & estimation of transformation efficiency
Learning outcomes
- ▸Plasmid isolation
- ▸Restriction digestion
- ▸Transformation
- ▸Gel electrophoresis
Lab component / notes
Supports MB304
Reference books
- •Agarwal KL
- •Glick HP
- •Cunningham WP