Semester V
MB304·Sem V·Theory
Recombinant DNA Technology
3
Credits
2-1-0
L-T-P
5
Units
Key topics
- ▸Restriction enzymes (Class I/II/III
- ▸star activity)
- ▸DNA modifying enzymes (ligase, phosphatase, RT)
- ▸Cloning vectors (plasmids, phage lambda, cosmids, YACs, BACs)
- ▸Gene libraries (genomic, cDNA)
- ▸Screening (colony hybridization, immunological)
- ▸DNA sequencing (Sanger, Maxam-Gilbert)
- ▸PCR types (conventional, RT-PCR, multiplex)
- ▸Gel electrophoresis (agarose, SDS-PAGE)
- ▸Applications (insulin, Hep B vaccine, growth hormone)
- ▸Ethical/social/environmental issues
Learning outcomes
- ▸Restriction enzymes
- ▸Cloning vectors
- ▸Gene library
- ▸PCR
- ▸Recombinant DNA applications
Lab component / notes
MB306 (Lab)
Reference books
- •Primrose & Twyman
- •Sambrook & Russell
- •Watson et al.