Semester V
MB303·Sem V·Theory
Cytogenetics and Molecular Diagnostics
3
Credits
2-1-0
L-T-P
5
Units
Key topics
- ▸Nucleic acid structure (DNA double helix, RNA types)
- ▸Gene expression (replication, transcription, translation)
- ▸Chromosomes & cytogenetic techniques (karyotyping, spectral karyotyping)
- ▸Chromosomal abnormalities
- ▸Molecular diagnostics (PCR, qPCR, blotting techniques, FISH)
- ▸Genetic disorder diagnosis (sickle cell, CML, thalassemia)
- ▸Body fluids
- ▸Stem cell banking & HLA typing
- ▸Nucleic acid purification, gel electrophoresis, gene therapy, microarrays, tumor markers
Learning outcomes
- ▸Nucleic acid structure
- ▸Chromosomal analysis
- ▸Molecular diagnostic techniques
- ▸Stem cell applications
Lab component / notes
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Reference books
- •Vasudevan
- •Chatterjee & Shinde
- •Lehninger
- •Ranjna Chawla